This page represents the latest version, and you may click to view the original version: Version 1.0

Basic Information:

Symbol	PDZD8
Synonyms	PDZK8
Protein Name	PDZ domain-containing protein 8 (Sarcoma antigen NY-SAR-84/NY-SAR-104)
Species	Human
Entrez ID	118987
Uniprot ID	Q8NEN9
Membrane Contact Site	ER-Endosome; Endosome-ER
Location (from literature)	ER
Cell line/Tissue	HEK293 cells; HeLa cells; HEK293T cells; COS-7 cells; U2-OS cells
Experimental Method	Low throughput experimental methods
Protein Sequence
More related results

Complex Information:

Complex ID	Subunit of complex	Subcellular location	Species	More
CMCS00018	PDZD8; RAB7A	ER-Endosome; Endosome-ER	Human	more
CMCS00183	PDZD8; RAB7A; ZFYVE27	ER-Endosome; Endosome-ER	Human	more

Expression Overview of PDZD8:

Homology Information of PDZD8:

Uniprot ID	Q8NEN9
EggNOG	KOG3532
HOGENOM	CLU_008594_0_0_1
OrthoDB	3681901at2759
TreeFam	TF324166
GeneTree	ENSGT00390000017746

References:

Pubmed ID	31636202
DOI	10.1073/pnas.1913509116
Description	PDZD8 mediates a Rab7-dependent interaction of the ER with late endosomes and lysosomes.
Description of experimental evidence	The protein was validated by microscopy, immunofluorescence and pull-down assay in HEK293 cells and HeLa cells.
More related results

Pubmed ID	33912962
DOI	10.1242/jcs.255026
Description	PDZD8-mediated lipid transfer at contacts between the ER and late endosomes/lysosomes is required for neurite outgrowth.
Description of experimental evidence	The protein was validated by confocal microscopy, live cell super-resolution Lattice-SIM microscopy, immunofluorescence staining, quantitative RT-PCR, GFP-trap assay, mass spectrometry, pulldown assays and pull-down assay in HeLa cells, HEK293T cells, COS-7 cells and U2-OS cells, which is required for LE/lys positioning and neurite outgrowth, which is dependent on the lipid transfer activity of the SMP domain.
More related results

Pubmed ID	32686675
DOI	10.1038/s41467-020-17451-7
Description	Our results identify the ER membrane proteins, PDZD8 and Protrudin, together with the late endosomal protein Rab7, as components of an ER-late endosome MCS in mammalian cells.
Description of experimental evidence	The protein was validated by immunofluorescence, spinning-disc confocal microscopy, immunopurifications, immunoprecipitation analysis and mass spectrometry analysis in HEK293T cells and U2-OS cells, which functions to regulate late endosomal motility and distribution, which is intimately linked to endosomal maturation.
More related results