Detail Information

P450 Protein:

P450 Symbol	CYP71D443
Protein Name	Cytochrome P450
Uniprot ID	A0A0M4U0E0
Species	Ajuga reptans (Bugle)
Txid	38596
P450 Protein Structure	Go to AlphaFold Website Model Confidence (pLDDT) Very high (>90) High (90 > 70) Low (70 > 50) Very low (<50) Powered by PDBe Molstar & AlphaFold
Subcellular Location	Endoplasmic reticulum membrane; Multi-pass membrane protein Microsome membrane; Multi-pass membrane protein
Protein Sequence	GO

Reaction Scheme:

(5aR,7S,9aR,11aR)-7-hydroxy-5a,9a,11a-trimethyl-1-[(2R)-6-methylhept-2-yl]hexadecahydro-1H-cyclopenta[1,2-a]phenanthren-5-one

CYP71D443

(5aR,7S,9aR,11aS)-7-hydroxy-1-[(2S,3R)-3-hydroxy-6-methylhept-2-yl]-5a,9a,11a-trimethylhexadecahydro-1H-cyclopenta[1,2-a]phenanthren-5-one

Substrate Information:

Substrate Name	(5aR,7S,9aR,11aR)-7-hydroxy-5a,9a,11a-trimethyl-1-[(2R)-6-methylhept-2-yl]hexadecahydro-1H-cyclopenta[1,2-a]phenanthren-5-one
Substrate Chemical Formula	C28H48O2
Substrate Smiles	C1C[C@@](C)2C3CC[C@@](C)4C([C@H](C)CCCC(C)C)CCC4C3CC(=O)[C@]2(C)C[C@H]1O
Substrate Structure	Download SDF

Product Information:

Product Name	(5aR,7S,9aR,11aS)-7-hydroxy-1-[(2S,3R)-3-hydroxy-6-methylhept-2-yl]-5a,9a,11a-trimethylhexadecahydro-1H-cyclopenta[1,2-a]phenanthren-5-one
Product Chemical Formula	C28H48O3
Product Smiles	C1C[C@@](C)2C3CC[C@@](C)4C([C@H](C)[C@H](O)CCC(C)C)CCC4C3CC(=O)[C@]2(C)C[C@H]1O
Product Structure	Download SDF

References:

Title:	Functional characterization of CYP71D443, a cytochrome P450 catalyzing C-22 hydroxylation in the 20-hydroxyecdysone biosynthesis of Ajuga hairy roots.
PMID:	27017303
Journal:	Phytochemistry
Year:	2016/7/1