Cytochromes P450 (CYPs) are enzymes that utilize heme as a cofactor to catalyze monooxygenase reactions. They belong to a superfamily of enzymes that have been identified in all kingdoms of life, including plants, animals, fungi, protists, bacteria, archaea, and even viruses. P450s are known for their remarkable ability to catalyze over 20 types of oxido-reduction reactions, making them "Nature's Most Versatile Biological Catalysts." However, despite the existence of more than 300,000 P450 genes in different databases, less than 0.2% of them have been functionally characterized.
    Here, we present the P450Reaction database, a manually curated resource of literature-supported reactions catalyzed by P450s. Representing a significant expansion from our previous dataset (1,600 reactions, 600 P450s, and 200 species), the current database catalogs over 3,700 reactions involving about 850 P450s from a wide range of over 400 species, including plants, animals, fungi, bacteria, and archaea.
    Our aim is to offer a useful platform for research in metabolism, synthetic biology, pharmacology, and enzyme engineering. By providing a comprehensive and reliable resource of P450 reactions, we hope to facilitate the discovery of new P450 functions, the design of new drugs, and the development of new biotechnologies.

Please cite us: P450Rdb: a manually curated database of reactions catalyzed by cytochrome P450 enzymes, Journal of advanced research, 2023, DOI: 10.1016/j.jare.2023.10.012