Detail Information
P450 Protein:
| P450 Symbol | Cyp96T1 |
| Protein Name | Noroxomaritidine synthase |
| Uniprot ID | A0A140IL90 |
| EC Number | 1.14.19.50 |
| Species | Narcissus aff. pseudonarcissus MK-2014 (Daffodil) |
| Txid | 1540222 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C16H19NO3 + C17H21N4O9P-2 + O2 → C16H18NO3+ + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH-OH |
| RheaID | 51264 |
+
+
Cyp96T1
+
+
+
Substrate Information:
| Substrate Name | 4′-O-Methylnorbelladine |
| Substrate Chemical Formula | C16H19NO3 |
| Substrate PubChem CID | 253994 |
| Substrate Smiles | COC1=C(C=C(C=C1)CNCCC2=CC=C(C=C2)O)O |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | (10Bs,4Ar)-Noroxomaritidine |
| Product Chemical Formula | C16H18NO3+ |
| Product PubChem CID | 122391295 |
| Product Smiles | COC1=C(C=C2C[NH+]3CC[C@@]4([C@H]3CC(=O)C=C4)C2=C1)O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | CYP96T1 of Narcissus sp. aff. pseudonarcissus Catalyzes Formation of the Para-Para' C-C Phenol Couple in the Amaryllidaceae Alkaloids. |
| PMID: | 26941773 |
| Journal: | Frontiers in plant science |
| Year: | 2016/1/1 |