Detail Information
P450 Protein:
| P450 Symbol | CYP5150L8 |
| Protein Name | Ganoderic acid synthetase CYP5150L8 |
| Uniprot ID | A0A2R4SBW0 |
| EC Number | 1.-.-.- |
| Species | Ganoderma lucidum (Ling zhi medicinal fungus) (Bracket fungus) |
| Txid | 5315 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C30H48O2 + C17H21N4O9P-2 + O2 → C30H47O3- + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH3 |
| RheaID | 68728 |
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CYP5150L8
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Substrate Information:
| Substrate Name | 26-Oxolanosterol |
| Substrate Chemical Formula | C30H48O2 |
| Substrate PubChem CID | 156587284 |
| Substrate Smiles | C[C@H](CC/C=C(C)/C=O)[C@H]1CC[C@@]2([C@@]1(CCC3=C2CC[C@@H]4[C@@]3(CC[C@@H](C4(C)C)O)C)C)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 3Beta-hydroxy-lanosta-8, 24-dien-26-oate |
| Product Chemical Formula | C30H47O3- |
| Product PubChem CID | 156587285 |
| Product Smiles | C[C@H](CC/C=C(C)/C(=O)[O-])[C@H]1CC[C@@]2([C@@]1(CCC3=C2CC[C@@H]4[C@@]3(CC[C@@H](C4(C)C)O)C)C)C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Biosynthesis of a ganoderic acid in Saccharomyces cerevisiae by expressing a cytochrome P450 gene from Ganoderma lucidum. |
| PMID: | 29476632 |
| Journal: | Biotechnology and bioengineering |
| Year: | 2018/7/1 |