Detail Information
P450 Protein:
| P450 Symbol | CYP75B137 |
| Protein Name | Flavonoid 3'-monooxygenase CYP75B137 |
| Uniprot ID | A0A4D6Q415 |
| EC Number | 1.14.14.82 |
| Species | Crocosmia x crocosmiiflora (Montbretia) (Crocosmia aurea x Crocosmia pottsii) |
| Txid | 1053288 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C15HO2R9 + C17H21N4O9P-2 + O2 → C15HO3R9 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 16337 |
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+
CYP75B137
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+
Substrate Information:
| Substrate Name | A 3′-Unsubstituted Flavone |
| Substrate Chemical Formula | C15HO2R9 |
| Substrate PubChem SID | 85338425 |
| Substrate Smiles | C1(=C(C(=C(C2=C1OC(=C(C2=O)*)C3=C(C(=C(C(=C3*)*)*)[H])*)*)*)*)* |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | A 3′-Hydroxyflavone |
| Product Chemical Formula | C15HO3R9 |
| Product PubChem SID | 162012076 |
| Product Smiles | Oc1c([*])c([*])c([*])c(c1[*])-c1oc2c([*])c([*])c([*])c([*])c2c(=O)c1[*] |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Flavonol Biosynthesis Genes and Their Use in Engineering the Plant Antidiabetic Metabolite Montbretin A. |
| PMID: | 31004005 |
| Journal: | Plant physiology |
| Year: | 2019/7/1 |