Detail Information

P450 Protein:

P450 Symbol	CYP90G4
Protein Name	Cholesterol 16,22-dihydroxylase CYP90G4
Uniprot ID	A0A517FNC5
EC Number	1.14.14.-
Species	Paris polyphylla (Daiswa polyphylla)
Txid	49666
P450 Protein Structure	Go to AlphaFold Website Model Confidence (pLDDT) Very high (>90) High (90 > 70) Low (70 > 50) Very low (<50) Powered by PDBe Molstar & AlphaFold
Subcellular Location	Membrane; Single-pass membrane protein
Protein Sequence	GO

Reaction Scheme:

Chemical Conversion	C27H46O3 → C27H44O3
Reaction Type	Oxidation
Chemical Bond	CH;OH

16S,22S-Dihydroxycholesterol

CYP90G4

(2S,4aR,4bS,6aS,6bR,7S,9aS,10aS,10bS)-4a,6a,7-trimethyl-8-(3-methylbutyl)-2,3,4,4a,4b,5,6,6a,6b,7,8,9a,10,10a,10b,11-hexadecahydro-1H-naphtho[2',1':4,5]indeno[2,1-b]furan-2,8-diol

Substrate Information:

Substrate Name	16S,22S-Dihydroxycholesterol
Substrate Chemical Formula	C27H46O3
Substrate PubChem CID	91013295
Substrate Smiles	C[C@@H]([C@H]1[C@H](C[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)O)C)C)O)[C@H](CCC(C)C)O
Substrate Structure	Download SDF

Product Information:

Product Name	(2S,4aR,4bS,6aS,6bR,7S,9aS,10aS,10bS)-4a,6a,7-trimethyl-8-(3-methylbutyl)-2,3,4,4a,4b,5,6,6a,6b,7,8,9a,10,10a,10b,11-hexadecahydro-1H-naphtho[2',1':4,5]indeno[2,1-b]furan-2,8-diol
Product Chemical Formula	C27H44O3
Product Smiles	OC1(O[C@@H]2[C@@]([H])([C@]3(CC[C@]([H])4[C@@]([H])(CC=C5C[C@H](CC[C@@]54C)O)[C@]3([H])C2)C)[C@@H]1C)CC[C@H](C)C
Product Structure	Download SDF

References:

Title:	Repeated evolution of cytochrome P450-mediated spiroketal steroid biosynthesis in plants.
PMID:	31324795
Journal:	Nature communications
Year:	2019/7/19