Detail Information

P450 Protein:

P450 SymbolCYP260A1
Protein NameCytochrome P450 CYP260A1
Uniprot IDA9FDB7
EC Number1.14.15.19
SpeciesSorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So ce56))
Txid 448385
P450 Protein Structure
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
Powered by PDBe Molstar & AlphaFold
Subcellular Location/
Protein Sequence GO

Reaction Scheme:

Chemical Conversion C19H28O2 + Fe2S2 + O2 + H+ → C19H28O3 + Fe2S2 + H2O
Reaction TypeOxidation
Chemical BondCH2
RheaID 50340

Testosterone

+

[2Fe-2S]1+

+

O2

+

H+

CYP260A1

1Alpha-Hydroxytestosterone

+

[2Fe-2S]2+

+

H2O

Substrate Information:

Substrate Name Testosterone
Substrate Chemical FormulaC19H28O2
Substrate PubChem CID6013
Substrate SmilesC[C@]12CC[C@H]3[C@H]([C@@H]1CC[C@@H]2O)CCC4=CC(=O)CC[C@]34C
Substrate Structure
Substrate Name [2Fe-2S]1+
Substrate Chemical FormulaFe2S2
Substrate SmilesS1[Fe]S[Fe+]1
Substrate Structure
Substrate Name O2
Substrate Chemical FormulaO2
Substrate PubChem CID977
Substrate CAS Number7782-44-7
Substrate SmilesO=O
Substrate Structure
Substrate Name H+
Substrate Chemical FormulaH+
Substrate PubChem CID1038
Substrate CAS Number12408-02-5
Substrate Smiles[H+]
Substrate Structure

Product Information:

Product Name 1Alpha-Hydroxytestosterone
Product Chemical FormulaC19H28O3
Product PubChem CID23256526
Product SmilesC[C@]12CC[C@H]3[C@H]([C@@H]1CC[C@@H]2O)CCC4=CC(=O)C[C@@H]([C@]34C)O
Product Structure
Product Name [2Fe-2S]2+
Product Chemical FormulaFe2S2
Product SmilesS1[Fe+]S[Fe+]1
Product Structure
Product Name H2O
Product Chemical FormulaH2O
Product PubChem CID962
Product CAS Number7732-18-5
Product SmilesO
Product Structure

References:

Title: Substrate Hunting for the Myxobacterial CYP260A1 Revealed New 1α-Hydroxylated Products from C-19 Steroids.
PMID: 26478560
Journal: Chembiochem : a European journal of chemical biology
Year: 2016/1/1

Contact zhy1001@alu.uestc.edu.cn or yangzhang@cdutcm.edu.cn
© Department of Bioinformatics