Detail Information
P450 Protein:
| P450 Symbol | TxtE |
| Protein Name | Putative P450-like protein |
| Uniprot ID | C9ZDC6 |
| Gene ID | 24308274 |
| Species | Streptomyces scabiei (strain 87.22) |
| Txid | 680198 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | / |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C11H12N2O2 + NO + C21H26N7O17P3-4 + O2 → C11H11N3O4 |
| Reaction Type | Oxidation |
| Chemical Bond | CH2 |
| Conditions | pH 8, 25°C |
+
+
+
TxtE
Substrate Information:
| Substrate Name | L-Tryptophan |
| Substrate Chemical Formula | C11H12N2O2 |
| Substrate PubChem CID | 6305 |
| Substrate Smiles | C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)O)N |
| Substrate Structure |
| Substrate Name | Nitric Oxide |
| Substrate Chemical Formula | NO |
| Substrate PubChem CID | 145068 |
| Substrate CAS Number | 10102-43-9 |
| Substrate Smiles | [N]=O |
| Substrate Structure |
| Substrate Name | NADPH(4-) |
| Substrate Chemical Formula | C21H26N7O17P3-4 |
| Substrate PubChem CID | 15983949 |
| Substrate Smiles | C1C=CN(C=C1C(=O)N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)([O-])OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)OP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | (2S)-3-(1H-indol-3-yl)-2-aminopropanoic acid |
| Product Chemical Formula | C11H11N3O4 |
| Product Smiles | C1=C([*]([*])=O)C2C(C[C@H](N)C(O)=O)=CNC=2C=C1 |
| Product Structure |
References:
| Title: | Cytochrome P450–catalyzed L-tryptophan nitration in thaxtomin phytotoxin biosynthesis. |
| PMID: | 22941045 |
| Journal: | Nature chemical biology |
| Year: | 2012/10/1 |