P450 Protein:

P450 Symbol	daf-9
Protein Name	3-ketosteroid oxygenase
Uniprot ID	H2KYS3
EC Number	1.14.14.-
Gene ID	180889
Species	Caenorhabditis elegans
Txid	6239
P450 Protein Structure	Go to AlphaFold Website Model Confidence (pLDDT) Very high (>90) High (90 > 70) Low (70 > 50) Very low (<50) Powered by PDBe Molstar & AlphaFold
Subcellular Location	Membrane; Multi-pass membrane protein
Protein Sequence	GO

Reaction Scheme:

Chemical Conversion	C27H48O3 → C27H45O5-
Reaction Type	Oxidation
Chemical Bond	CH3

Substrate Information:

Substrate Name	5Beta-Cholestane-3Alpha,7Alpha,12Alpha-Triol
Substrate Chemical Formula	C27H48O3
Substrate PubChem CID	160520
Substrate Smiles	C[C@H](CCCC(C)C)[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C
Substrate Structure	Download SDF

Product Information:

Product Name	(25R)-3Alpha,7Alpha,12Alpha-Trihydroxy-5Beta-Cholestan-26-Oate
Product Chemical Formula	C27H45O5-
Product PubChem CID	45266747
Product Smiles	C[C@H](CCC[C@@H](C)C(=O)[O-])[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C
Product Structure	Download SDF

References: