Detail Information
P450 Protein:
| P450 Symbol | CYP82X1 |
| Protein Name | (13S,14R)-13-O-acetyl-1-hydroxy-N-methylcanadine 8-hydroxylase CYP82X1 |
| Uniprot ID | I3V6B7 |
| EC Number | 1.14.14.167 |
| Species | Papaver somniferum (Opium poppy) |
| Txid | 3469 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | / |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C23H26NO7+ + C17H21N4O9P-2 + O2 → C23H26NO8+ + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH2 |
| RheaID | 57388 |
+
+
CYP82X1
+
+
+
Substrate Information:
| Substrate Name | (13S,14R)-13-O-Acetyl-1-Hydroxy-N-Methylcanadine |
| Substrate Chemical Formula | C23H26NO7+ |
| Substrate PubChem CID | 86278052 |
| Substrate Smiles | CC(=O)O[C@@H]1[C@H]2C3=C(C4=C(C=C3CC[N+]2(CC5=C1C=CC(=C5OC)OC)C)OCO4)O |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | (13S,14R)-13-O-Acetyl-1,8-Dihydroxy-N-Methylcanadine |
| Product Chemical Formula | C23H26NO8+ |
| Product PubChem CID | 129011757 |
| Product Smiles | CC(=O)O[C@@H]1[C@H]2C3=C(C4=C(C=C3CC[N+]2(C(C5=C1C=CC(=C5OC)OC)O)C)OCO4)O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Acetylation serves as a protective group in noscapine biosynthesis in opium poppy. |
| PMID: | 25485687 |
| Journal: | Nature chemical biology |
| Year: | 2015/2/1 |
| Title: | Engineering biosynthesis of the anticancer alkaloid noscapine in yeast. |
| PMID: | 27378283 |
| Journal: | Nature communications |
| Year: | 2016/7/5 |