Detail Information
P450 Protein:
| P450 Symbol | CYP8B1 |
| Protein Name | 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase |
| Uniprot ID | O02766 |
| EC Number | 1.14.14.139 |
| Gene ID | 100009301 |
| Species | Oryctolagus cuniculus (Rabbit) |
| Txid | 9986 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Endoplasmic reticulum membrane; Single-pass membrane protein Microsome membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C24H40O4 + C17H21N4O9P-2 + O2 → C24H40O5 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH2 |
| RheaID | 65700 |
+
+
CYP8B1
+
+
+
Substrate Information:
| Substrate Name | Chenodeoxycholate |
| Substrate Chemical Formula | C24H40O4 |
| Substrate PubChem CID | 10133 |
| Substrate Smiles | C[C@H](CCC(=O)O)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | Cholate |
| Product Chemical Formula | C24H40O5 |
| Product PubChem CID | 221493 |
| Product Smiles | C[C@H](CCC(=O)O)[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Purification and characterization of 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase. |
| PMID: | 1400444 |
| Journal: | The Journal of biological chemistry |
| Year: | 1992/10/25 |
| Title: | Molecular cloning and expression of rabbit sterol 12alpha-hydroxylase. |
| PMID: | 8943286 |
| Journal: | The Journal of biological chemistry |
| Year: | 1996/12/13 |