Detail Information
P450 Protein:
| P450 Symbol | CYP75A7 |
| Protein Name | Flavonoid 3',5'-hydroxylase |
| Uniprot ID | O04790 |
| EC Number | 1.14.14.81 |
| Species | Eustoma exaltatum subsp. russellianum (Bluebells) (Eustoma grandiflorum) |
| Txid | 52518 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | / |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C15H2O2R10 + C17H21N4O9P-2 + O2 → C15H2O4R10 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| RheaID | 55448 |
+
+
CYP75A7
+
+
+
Substrate Information:
| Substrate Name | A 3′,5′-Unsubstituted Flavanone |
| Substrate Chemical Formula | C15H2O2R10 |
| Substrate Smiles | O1C2=C(C(C(C1(C3=C(C=C(C=C3*)*)*)*)(*)*)=O)C(=C(C(=C2*)*)*)* |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | A 3′,5′-Dihydroxyflavanone |
| Product Chemical Formula | C15H2O4R10 |
| Product PubChem SID | 348353755 |
| Product Smiles | O1C2=C(C(C(C1(C3=C(C(=C(C(=C3*)O)*)O)*)*)(*)*)=O)C(=C(C(=C2*)*)*)* |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Expression of chimeric P450 genes encoding flavonoid-3', 5'-hydroxylase in transgenic tobacco and petunia plants(1). |
| PMID: | 10567704 |
| Journal: | FEBS letters |
| Year: | 1999/11/19 |