Detail Information

P450 Protein:

P450 Symbolcyp134
Protein NamePulcherriminic acid synthase
Uniprot IDO34926
EC Number1.14.15.13
Gene ID 936624
SpeciesBacillus subtilis (strain 168)
Txid 224308
P450 Protein Structure
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
Powered by PDBe Molstar & AlphaFold
Subcellular Location/
Protein Sequence GO

Reaction Scheme:

Chemical Conversion C12H22N2O2 + Fe2S2 + O2 + H+ → C12H20N2O4 + Fe2S2 + H2O
Reaction TypeOxidation
Chemical BondNH-CH;-NH-
RheaID 35555

Cyclo(L-Leucyl-L-Leucyl)

+

[2Fe-2S]1+

+

O2

+

H+

cyp134

Pulcherriminic Acid

+

[2Fe-2S]2+

+

H2O

Substrate Information:

Substrate Name Cyclo(L-Leucyl-L-Leucyl)
Substrate Chemical FormulaC12H22N2O2
Substrate PubChem CID192731
Substrate SmilesCC(C)C[C@H]1C(=O)N[C@H](C(=O)N1)CC(C)C
Substrate Structure
Substrate Name [2Fe-2S]1+
Substrate Chemical FormulaFe2S2
Substrate SmilesS1[Fe]S[Fe+]1
Substrate Structure
Substrate Name O2
Substrate Chemical FormulaO2
Substrate PubChem CID977
Substrate CAS Number7782-44-7
Substrate SmilesO=O
Substrate Structure
Substrate Name H+
Substrate Chemical FormulaH+
Substrate PubChem CID1038
Substrate CAS Number12408-02-5
Substrate Smiles[H+]
Substrate Structure

Product Information:

Product Name Pulcherriminic Acid
Product Chemical FormulaC12H20N2O4
Product PubChem CID3083664
Product SmilesCC(C)CC1=C([N+](=C(C(=O)N1O)CC(C)C)[O-])O
Product Structure
Product Name [2Fe-2S]2+
Product Chemical FormulaFe2S2
Product SmilesS1[Fe+]S[Fe+]1
Product Structure
Product Name H2O
Product Chemical FormulaH2O
Product PubChem CID962
Product CAS Number7732-18-5
Product SmilesO
Product Structure

References:

Title: Structural and biochemical characterization of the cytochrome P450 CypX (CYP134A1) from Bacillus subtilis: a cyclo-L-leucyl-L-leucyl dipeptide oxidase.
PMID: 20690619
Journal: Biochemistry
Year: 2010/8/31

Contact zhy1001@alu.uestc.edu.cn or yangzhang@cdutcm.edu.cn
© Department of Bioinformatics