Detail Information
P450 Protein:
| P450 Symbol | claM |
| Protein Name | Cytochrome P450 monooxygenase claM |
| Uniprot ID | P0CU70 |
| EC Number | 1.14.13.- |
| Species | Passalora fulva (Tomato leaf mold) (Cladosporium fulvum) |
| Txid | 5499 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C15H10O5 + C17H21N4O9P-2 + O2 → C30H18O10 + FMN + H2O + H+ |
| Reaction Type | Combination |
| Chemical Bond | CH |
| RheaID | 64288 |
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claM
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Substrate Information:
| Substrate Name | Nataloe Emodin |
| Substrate Chemical Formula | C15H10O5 |
| Substrate PubChem CID | 12313148 |
| Substrate Smiles | CC1=CC2=C(C(=C1)O)C(=O)C3=C(C2=O)C=CC(=C3O)O |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | Cladofulvin |
| Product Chemical Formula | C30H18O10 |
| Product PubChem CID | 71434670 |
| Product Smiles | CC1=CC2=C(C(=C1C3=C4C(=C(C=C3C)O)C(=O)C5=C(C4=O)C=CC(=C5O)O)O)C(=O)C6=C(C2=O)C=CC(=C6O)O |
| Product Structure |
| Product Name | FMN |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Elucidation of cladofulvin biosynthesis reveals a cytochrome P450 monooxygenase required for anthraquinone dimerization. |
| PMID: | 27274078 |
| Journal: | Proceedings of the National Academy of Sciences of the United States of America |
| Year: | 2016/6/21 |