Detail Information
P450 Protein:
| P450 Symbol | gcoA |
| Protein Name | Aromatic O-demethylase, cytochrome P450 subunit |
| Uniprot ID | P0DPQ7 |
| EC Number | 1.14.14.- |
| Species | Amycolatopsis sp. (strain ATCC 39116 / 75iv2) |
| Txid | 385957 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | / |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C8H10O2 + C27H33N9O15P2-2 + O2 → C6H6O2 + 75-07-0 + C27H30N9O15P2-3 + H2O + H+ |
| Reaction Type | Decomposition |
| Chemical Bond | C-O |
| RheaID | 57436 |
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+
gcoA
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+
+
+
Substrate Information:
| Substrate Name | Guaethol |
| Substrate Chemical Formula | C8H10O2 |
| Substrate PubChem CID | 66755 |
| Substrate Smiles | CCOC1=CC=CC=C1O |
| Substrate Structure |
| Substrate Name | FADH2 |
| Substrate Chemical Formula | C27H33N9O15P2-2 |
| Substrate PubChem CID | 46931118 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])OP(=O)([O-])OC[C@@H]4[C@H]([C@H]([C@@H](O4)N5C=NC6=C(N=CN=C65)N)O)O)O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | Catechol |
| Product Chemical Formula | C6H6O2 |
| Product PubChem CID | 289 |
| Product Smiles | C1=CC=C(C(=C1)O)O |
| Product Structure |
| Product Name | Acetaldehyde |
| Product Chemical Formula | 75-07-0 |
| Product PubChem CID | 177 |
| Product Smiles | CC=O |
| Product Structure |
| Product Name | FAD |
| Product Chemical Formula | C27H30N9O15P2-3 |
| Product PubChem CID | 46906035 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])OP(=O)([O-])OC[C@@H]4[C@H]([C@H]([C@@H](O4)N5C=NC6=C(N=CN=C65)N)O)O)O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion. |
| PMID: | 29950589 |
| Journal: | Nature communications |
| Year: | 2018/6/27 |