Detail Information
P450 Protein:
| P450 Symbol | NCP1 |
| Protein Name | NADPH--cytochrome P450 reductase |
| Uniprot ID | P16603 |
| EC Number | 1.6.2.4 |
| Gene ID | 856438 |
| Species | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
| Txid | 559292 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side Mitochondrion outer membrane; Single-pass membrane protein; Cytoplasmic side Cell membrane; Single-pass membrane protein; Cytoplasmic side |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C34H30FeN4O4- + C21H26N7O17P3-4 → C34H30FeN4O4-2 + C21H25N7O17P3-3 + H+ |
| Reaction Type | Reduction |
| Chemical Bond | Heme |
| RheaID | 24040 |
+
NCP1
+
+
Substrate Information:
| Substrate Name | Fe(III)-heme b |
| Substrate Chemical Formula | C34H30FeN4O4- |
| Substrate Smiles | CC1=C(CCC([O-])=O)C2=[N+]3C1=Cc1c(C)c(C=C)c4C=C5C(C)=C(C=C)C6=[N+]5[Fe-]3(n14)n1c(=C6)c(C)c(CCC([O-])=O)c1=C2 |
| Substrate Structure |
| Substrate Name | NADPH(4-) |
| Substrate Chemical Formula | C21H26N7O17P3-4 |
| Substrate PubChem CID | 15983949 |
| Substrate Smiles | C1C=CN(C=C1C(=O)N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)([O-])OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)OP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
Product Information:
| Product Name | heme b |
| Product Chemical Formula | C34H30FeN4O4-2 |
| Product Smiles | CC1=C(CCC([O-])=O)C2=[N+]3C1=Cc1c(C)c(C=C)c4C=C5C(C)=C(C=C)C6=[N+]5[Fe--]3(n14)n1c(=C6)c(C)c(CCC([O-])=O)c1=C2 |
| Product Structure |
| Product Name | NADP+ |
| Product Chemical Formula | C21H25N7O17P3-3 |
| Product PubChem CID | 162422398 |
| Product Smiles | C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)([O-])OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)O)OP(=O)([O-])[O-])O)O)C(=O)N |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Activities and kinetic mechanisms of native and soluble NADPH-cytochrome P450 reductase. |
| PMID: | 11485306 |
| Journal: | Biochemical and biophysical research communications |
| Year: | 2001/8/10 |
| Title: | Cytochrome P-450 reductase is responsible for the ferrireductase activity associated with isolated plasma membranes of Saccharomyces cerevisiae. |
| PMID: | 9368374 |
| Journal: | FEMS microbiology letters |
| Year: | 1997/11/1 |
| Title: | The N-terminal membrane domain of yeast NADPH-cytochrome P450 (CYP) oxidoreductase is not required for catalytic activity in sterol biosynthesis or in reconstitution of CYP activity. |
| PMID: | 9468503 |
| Journal: | The Journal of biological chemistry |
| Year: | 1998/2/20 |