Detail Information
P450 Protein:
| P450 Symbol | CYP107H |
| Protein Name | Biotin biosynthesis cytochrome P450 |
| Uniprot ID | P53554 |
| EC Number | 1.14.14.46 |
| Gene ID | 935928 |
| Species | Bacillus subtilis (strain 168) |
| Txid | 224308 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | / |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C22H38N3O9PSR + C17H21N4O9P-2 + O2 → C21H34N3O11PS + CHOR + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Decomposition |
| Chemical Bond | CH2-R |
| RheaID | 52852 |
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CYP107H
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+
+
+
Substrate Information:
| Substrate Name | A C2-C8-Saturated Long-Chain Fatty Acyl-[Acp] |
| Substrate Chemical Formula | C22H38N3O9PSR |
| Substrate Structure |
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|---|
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 6-Carboxyhexanoyl-[Acp] |
| Product Chemical Formula | C21H34N3O11PS |
| Product PubChem SID | 223444102 |
| Product Smiles | CC(C)(COP([O-])(=O)OC[C@H](N-*)C(-*)=O)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)CCCCCC([O-])=O |
| Product Structure |
| Product Name | A Fatty Aldehyde |
| Product Chemical Formula | CHOR |
| Product PubChem SID | 252149716 |
| Product Smiles | [*]C=O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Expression, purification, and characterization of BioI: a carbon-carbon bond cleaving cytochrome P450 involved in biotin biosynthesis in Bacillus subtilis. |
| PMID: | 11368323 |
| Journal: | Archives of biochemistry and biophysics |
| Year: | 2000/12/15 |