Detail Information
P450 Protein:
| P450 Symbol | CYP27A1 |
| Protein Name | Sterol 26-hydroxylase, mitochondrial |
| Uniprot ID | Q02318 |
| EC Number | 1.14.15.15 |
| Gene ID | 1593 |
| Species | Homo sapiens (Human) |
| Txid | 9606 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Mitochondrion inner membrane; Peripheral membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C27H46O + Fe2S2 + O2 + H+ → C27H46O2 + Fe2S2 + H2O |
| Reaction Type | Oxidation |
| Chemical Bond | CH3 |
| RheaID | 46400 |
+
+
+
CYP27A1
+
+
Substrate Information:
| Substrate Name | Cholesterol |
| Substrate Chemical Formula | C27H46O |
| Substrate PubChem CID | 5997 |
| Substrate Smiles | C[C@H](CCCC(C)C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)O)C)C |
| Substrate Structure |
| Substrate Name | [2Fe-2S]1+ |
| Substrate Chemical Formula | Fe2S2 |
| Substrate Smiles | S1[Fe]S[Fe+]1 |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
| Substrate Name | H+ |
| Substrate Chemical Formula | H+ |
| Substrate PubChem CID | 1038 |
| Substrate CAS Number | 12408-02-5 |
| Substrate Smiles | [H+] |
| Substrate Structure |
Product Information:
| Product Name | 27-Hydroxycholesterol |
| Product Chemical Formula | C27H46O2 |
| Product PubChem CID | 123976 |
| Product Smiles | C[C@H](CCC[C@@H](C)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)O)C)C)CO |
| Product Structure |
| Product Name | [2Fe-2S]2+ |
| Product Chemical Formula | Fe2S2 |
| Product Smiles | S1[Fe+]S[Fe+]1 |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
References:
| Title: | Metabolism of 4 beta -hydroxycholesterol in humans. |
| PMID: | 12077124 |
| Journal: | The Journal of biological chemistry |
| Year: | 2002/8/30 |
| Title: | Conversion of 7-ketocholesterol to oxysterol metabolites by recombinant CYP27A1 and retinal pigment epithelial cells. |
| PMID: | 21411718 |
| Journal: | Journal of lipid research |
| Year: | 2011/6/1 |
| Title: | Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways. |
| PMID: | 9660774 |
| Journal: | The Journal of biological chemistry |
| Year: | 1998/7/17 |