Detail Information
P450 Protein:
| P450 Symbol | CYP71AV1 |
| Protein Name | Amorpha-4,11-diene 12-monooxygenase |
| Uniprot ID | Q1PS23 |
| EC Number | 1.14.14.114 |
| Species | Artemisia annua (Sweet wormwood) |
| Txid | 35608 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C15H24 + C17H21N4O9P-2 + O2 → C15H21O2- + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH3 |
| RheaID | 32999 |
+
+
CYP71AV1
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+
+
Substrate Information:
| Substrate Name | (+)-Amorpha-4,11-Diene |
| Substrate Chemical Formula | C15H24 |
| Substrate PubChem CID | 11052747 |
| Substrate Smiles | C[C@@H]1CC[C@H]([C@@H]2[C@H]1CCC(=C2)C)C(=C)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | (+)-Artemisinate |
| Product Chemical Formula | C15H21O2- |
| Product PubChem CID | 52940136 |
| Product Smiles | C[C@@H]1CC[C@H]([C@@H]2[C@H]1CCC(=C2)C)C(=C)C(=O)[O-] |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin. |
| PMID: | 16458889 |
| Journal: | FEBS letters |
| Year: | 2006/2/20 |
| Title: | Production of the antimalarial drug precursor artemisinic acid in engineered yeast. |
| PMID: | 16612385 |
| Journal: | Nature |
| Year: | 2006/4/13 |
| Title: | Comparative functional analysis of CYP71AV1 natural variants reveals an important residue for the successive oxidation of amorpha-4,11-diene. |
| PMID: | 23246612 |
| Journal: | FEBS letters |
| Year: | 2013/1/31 |