Detail Information
P450 Protein:
| P450 Symbol | Cyp7b1 |
| Protein Name | 25-hydroxycholesterol 7-alpha-hydroxylase |
| Uniprot ID | Q63688 |
| EC Number | 1.14.14.29 |
| Gene ID | 25429 |
| Species | Rattus norvegicus (Rat) |
| Txid | 10116 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Mitochondrion |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C27H46O2 + C17H21N4O9P-2 + O2 → C27H46O3 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH2 |
| RheaID | 24308 |
+
+
Cyp7b1
+
+
+
Substrate Information:
| Substrate Name | 25-Hydroxycholesterol |
| Substrate Chemical Formula | C27H46O2 |
| Substrate PubChem CID | 65094 |
| Substrate Smiles | C[C@H](CCCC(C)(C)O)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2CC=C4[C@@]3(CC[C@@H](C4)O)C)C |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 7Alpha,25-Dihydroxycholesterol |
| Product Chemical Formula | C27H46O3 |
| Product PubChem CID | 11954197 |
| Product Smiles | C[C@H](CCCC(C)(C)O)[C@H]1CC[C@@H]2[C@@]1(CC[C@H]3[C@H]2[C@@H](C=C4[C@@]3(CC[C@@H](C4)O)C)O)C |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat. |
| PMID: | 12759897 |
| Journal: | Metabolism: clinical and experimental |
| Year: | 2003/5/1 |