Detail Information
P450 Protein:
| P450 Symbol | CYP71E7 |
| Protein Name | 2-methylbutanal oxime monooxygenase |
| Uniprot ID | Q6XQ14 |
| EC Number | 1.14.14.41 |
| Species | Manihot esculenta (Cassava) (Jatropha manihot) |
| Txid | 3983 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Endoplasmic reticulum membrane; Single-pass type II membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C5H11NO + C17H21N4O9P-2 + O2 → C5H9NO + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | N-OH;CH |
| RheaID | 28422 |
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CYP71E7
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+
Substrate Information:
| Substrate Name | (1E,2S)-2-Methylbutanal Oxime |
| Substrate Chemical Formula | C5H11NO |
| Substrate PubChem CID | 12917320 |
| Substrate Smiles | CC[C@H](C)/C=N/O |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 2-Hydroxy-2-Methylbutanenitrile |
| Product Chemical Formula | C5H9NO |
| Product PubChem CID | 97823 |
| Product Smiles | CCC(C)(C#N)O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in cassava: isolation, biochemical characterization, and expression pattern of CYP71E7, the oxime-metabolizing cytochrome P450 enzyme. |
| PMID: | 21045121 |
| Journal: | Plant physiology |
| Year: | 2011/1/1 |