Detail Information
P450 Protein:
| P450 Symbol | cyp28 |
| Protein Name | Pentalenic acid synthase |
| Uniprot ID | Q825I8 |
| EC Number | 1.14.15.11 |
| Species | Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) |
| Txid | 227882 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Endoplasmic reticulum membrane; Multi-pass membrane protein Microsome membrane; Multi-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C15H21O2- + Fe4S4 + O2 + H+ → C15H21O3- + Fe4S4 + H2O |
| Reaction Type | Oxidation |
| Chemical Bond | CH2 |
| RheaID | 34499 |
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+
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cyp28
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+
Substrate Information:
| Substrate Name | 1-Deoxypentalenate |
| Substrate Chemical Formula | C15H21O2- |
| Substrate PubChem CID | 70678894 |
| Substrate Smiles | C[C@@H]1CC[C@@H]2[C@]13CC(C[C@H]3C=C2C(=O)[O-])(C)C |
| Substrate Structure |
| Substrate Name | [4Fe-4S]1+ cluster |
| Substrate Chemical Formula | Fe4S4 |
| Substrate Smiles | [S]12[Fe]3[S]4[Fe]1[S]1[Fe]2[S]3[Fe+]41 |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
| Substrate Name | H+ |
| Substrate Chemical Formula | H+ |
| Substrate PubChem CID | 1038 |
| Substrate CAS Number | 12408-02-5 |
| Substrate Smiles | [H+] |
| Substrate Structure |
Product Information:
| Product Name | Pentalenate |
| Product Chemical Formula | C15H21O3- |
| Product PubChem CID | 70678937 |
| Product Smiles | C[C@@H]1CC[C@@H]2[C@]13CC([C@@H]([C@H]3C=C2C(=O)[O-])O)(C)C |
| Product Structure |
| Product Name | [4Fe-4S]2+ cluster |
| Product Chemical Formula | Fe4S4 |
| Product Smiles | [S]12[Fe]3[S]4[Fe]1[S]1[Fe+]2[S]3[Fe+]41 |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
References:
| Title: | Pentalenic acid is a shunt metabolite in the biosynthesis of the pentalenolactone family of metabolites: hydroxylation of 1-deoxypentalenic acid mediated by CYP105D7 (SAV_7469) of Streptomyces avermitilis. |
| PMID: | 21081950 |
| Journal: | The Journal of antibiotics |
| Year: | 2011/1/1 |