Detail Information

P450 Protein:

P450 SymbolncsB3
Protein Name2-hydroxy-5-methyl-1-naphthoate 7-hydroxylase
Uniprot IDQ84HB6
EC Number1.14.15.31
SpeciesStreptomyces carzinostaticus
Txid 1897
P450 Protein Structure
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
Powered by PDBe Molstar & AlphaFold
Subcellular LocationMembrane; Single-pass membrane protein
Protein Sequence GO

Reaction Scheme:

Chemical Conversion C12H9O3- + Fe2S2 + O2 + H+ → C12H9O4- + Fe2S2 + H2O
Reaction TypeOxidation
Chemical BondCH
RheaID 42828

2-Hydroxy-5-Methyl-1-Naphthoate

+

[2Fe-2S]1+

+

O2

+

H+

ncsB3

2,7-Dihydroxy-5-Methyl-1-Naphthoate

+

[2Fe-2S]2+

+

H2O

Substrate Information:

Substrate Name 2-Hydroxy-5-Methyl-1-Naphthoate
Substrate Chemical FormulaC12H9O3-
Substrate PubChem CID86289942
Substrate SmilesCC1=C2C=CC(=C(C2=CC=C1)C(=O)O)[O-]
Substrate Structure
Substrate Name [2Fe-2S]1+
Substrate Chemical FormulaFe2S2
Substrate SmilesS1[Fe]S[Fe+]1
Substrate Structure
Substrate Name O2
Substrate Chemical FormulaO2
Substrate PubChem CID977
Substrate CAS Number7782-44-7
Substrate SmilesO=O
Substrate Structure
Substrate Name H+
Substrate Chemical FormulaH+
Substrate PubChem CID1038
Substrate CAS Number12408-02-5
Substrate Smiles[H+]
Substrate Structure

Product Information:

Product Name 2,7-Dihydroxy-5-Methyl-1-Naphthoate
Product Chemical FormulaC12H9O4-
Product PubChem CID86289503
Product SmilesCC1=CC(=CC2=C1C=CC(=C2C(=O)O)[O-])O
Product Structure
Product Name [2Fe-2S]2+
Product Chemical FormulaFe2S2
Product SmilesS1[Fe+]S[Fe+]1
Product Structure
Product Name H2O
Product Chemical FormulaH2O
Product PubChem CID962
Product CAS Number7732-18-5
Product SmilesO
Product Structure

References:

Title: In vivo characterization of NcsB3 to establish the complete biosynthesis of the naphthoic acid moiety of the neocarzinostatin chromophore.
PMID: 20735485
Journal: FEMS microbiology letters
Year: 2010/10/1

Contact zhy1001@alu.uestc.edu.cn or yangzhang@cdutcm.edu.cn
© Department of Bioinformatics