Detail Information
P450 Protein:
| P450 Symbol | CYP4F21 |
| Protein Name | Prostaglandin E2 omega-hydroxylase CYP4F21 |
| Uniprot ID | Q9GLL1 |
| EC Number | 1.14.14.- |
| Gene ID | 443099 |
| Species | Ovis aries (Sheep) |
| Txid | 9940 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Endoplasmic reticulum membrane; Single-pass type I membrane protein Microsome membrane; Single-pass type I membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C20H32O5 + C17H21N4O9P-2 + O2 → C20H32O6 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH3 |
| RheaID | 52488 |
+
+
CYP4F21
+
+
+
Substrate Information:
| Substrate Name | Prostaglandin E2 |
| Substrate Chemical Formula | C20H32O5 |
| Substrate PubChem CID | 5280360 |
| Substrate Smiles | CCCCC[C@@H](/C=C/[C@H]1[C@@H](CC(=O)[C@@H]1C/C=CCCCC(=O)O)O)O |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 20-Hydroxy Prostaglandin E2 |
| Product Chemical Formula | C20H32O6 |
| Product PubChem CID | 5283034 |
| Product Smiles | C1[C@H]([C@@H]([C@H](C1=O)C/C=CCCCC(=O)O)/C=C/[C@H](CCCCCO)O)O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Cloning and characterization of CYP4F21: a prostaglandin E2 20-hydroxylase of ram seminal vesicles. |
| PMID: | 11370662 |
| Journal: | Archives of biochemistry and biophysics |
| Year: | 2001/5/1 |