Detail Information
P450 Protein:
| P450 Symbol | CYP71B15 |
| Protein Name | Bifunctional dihydrocamalexate synthase/camalexin synthase |
| Uniprot ID | Q9LW27 |
| EC Number | 1.14.19.52 |
| Gene ID | 822298 |
| Species | Arabidopsis thaliana (Mouse-ear cress) |
| Txid | 3702 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | / |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C13H13N3O2S + C17H21N4O9P-2 + O2 → C11H8N2S + CHN + C17H18N4O9P-3 + CO2 + H2O + H+ |
| Reaction Type | Oxidation;Decomposition |
| Chemical Bond | CH-NH3(+);C-CH |
| RheaID | 52776 |
+
+
CYP71B15
+
+
+
+
+
Substrate Information:
| Substrate Name | 2-(L-Cystein-S-Yl)-2-(1H-Indol-3-Yl)-Acetonitrile |
| Substrate Chemical Formula | C13H13N3O2S |
| Substrate PubChem CID | 86289810 |
| Substrate Smiles | C1=CC=C2C(=C1)C(=CN2)C(C#N)SC[C@@H](C(=O)[O-])[NH3+] |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | Camalexin |
| Product Chemical Formula | C11H8N2S |
| Product PubChem CID | 636970 |
| Product Smiles | C1=CC=C2C(=C1)C(=CN2)C3=NC=CS3 |
| Product Structure |
| Product Name | hydrogen cyanide |
| Product Chemical Formula | CHN |
| Product PubChem CID | 768 |
| Product Smiles | C#N |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | CO2 |
| Product Chemical Formula | CO2 |
| Product PubChem CID | 280 |
| Product CAS Number | 124-38-9 |
| Product Smiles | O=C=O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis. |
| PMID: | 16766671 |
| Journal: | Plant physiology |
| Year: | 2006/8/1 |
| Title: | The multifunctional enzyme CYP71B15 (PHYTOALEXIN DEFICIENT3) converts cysteine-indole-3-acetonitrile to camalexin in the indole-3-acetonitrile metabolic network of Arabidopsis thaliana. |
| PMID: | 19567706 |
| Journal: | The Plant cell |
| Year: | 2009/6/1 |