Detail Information
P450 Protein:
| P450 Symbol | CYP79D1 |
| Protein Name | Valine N-monooxygenase 1 |
| Uniprot ID | Q9M7B8 |
| EC Number | 1.14.14.38; 1.14.14.39 |
| Species | Manihot esculenta (Cassava) (Jatropha manihot) |
| Txid | 3983 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Microsome membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C6H13NO2 + C17H21N4O9P-2 + O2 → C5H11NO + C17H18N4O9P-3 + CO2 + H2O + H+ |
| Reaction Type | Oxidoreduction |
| Chemical Bond | CH-NH2;Carboxyl |
| RheaID | 28602 |
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CYP79D1
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Substrate Information:
| Substrate Name | L-Isoleucine |
| Substrate Chemical Formula | C6H13NO2 |
| Substrate PubChem CID | 6306 |
| Substrate Smiles | CC[C@H](C)[C@@H](C(=O)O)N |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | (1E,2S)-2-Methylbutanal Oxime |
| Product Chemical Formula | C5H11NO |
| Product PubChem CID | 12917320 |
| Product Smiles | CC[C@H](C)/C=N/O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | CO2 |
| Product Chemical Formula | CO2 |
| Product PubChem CID | 280 |
| Product CAS Number | 124-38-9 |
| Product Smiles | O=C=O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin. Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes. |
| PMID: | 10636899 |
| Journal: | The Journal of biological chemistry |
| Year: | 2000/1/21 |