Detail Information
P450 Protein:
| P450 Symbol | CYP94B3 |
| Protein Name | Cytochrome P450 94B3 |
| Uniprot ID | Q9SMP5 |
| EC Number | 1.14.14.48 |
| Gene ID | 824011 |
| Species | Arabidopsis thaliana (Mouse-ear cress) |
| Txid | 3702 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Membrane; Single-pass membrane protein Endoplasmic reticulum membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C18H28NO4- + C21H26N7O17P3-4 + O2 + H+ → C18H28NO5- + C21H25N7O17P3-3 + H2O |
| Reaction Type | Oxidation |
| Chemical Bond | CH3 |
| RheaID | 54808 |
+
+
+
CYP94B3
+
+
Substrate Information:
| Substrate Name | L-Isoleucine-(+)-7-Isojasmonate |
| Substrate Chemical Formula | C18H28NO4- |
| Substrate PubChem CID | 54758681 |
| Substrate Smiles | CC/C=CC[C@H]1[C@H](CCC1=O)CC(=O)N[C@@H]([C@@H](C)CC)C(=O)[O-] |
| Substrate Structure |
| Substrate Name | NADPH(4-) |
| Substrate Chemical Formula | C21H26N7O17P3-4 |
| Substrate PubChem CID | 15983949 |
| Substrate Smiles | C1C=CN(C=C1C(=O)N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)([O-])OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)OP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
| Substrate Name | H+ |
| Substrate Chemical Formula | H+ |
| Substrate PubChem CID | 1038 |
| Substrate CAS Number | 12408-02-5 |
| Substrate Smiles | [H+] |
| Substrate Structure |
Product Information:
| Product Name | L-Isoleucine-(+)-12-Hydroxy-7-Isojasmonate |
| Product Chemical Formula | C18H28NO5- |
| Product PubChem CID | 126456509 |
| Product Smiles | CC[C@H](C)[C@@H](C(=O)[O-])NC(=O)C[C@H]1CCC(=O)[C@H]1C/C=CCCO |
| Product Structure |
| Product Name | NADP+ |
| Product Chemical Formula | C21H25N7O17P3-3 |
| Product PubChem CID | 162422398 |
| Product Smiles | C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)([O-])OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)O)OP(=O)([O-])[O-])O)O)C(=O)N |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
References:
| Title: | Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine. |
| PMID: | 21576464 |
| Journal: | Proceedings of the National Academy of Sciences of the United States of America |
| Year: | 2011/5/31 |