Detail Information
P450 Protein:
| P450 Symbol | CYP93C2 |
| Protein Name | 2-hydroxyisoflavanone synthase |
| Uniprot ID | Q9SXS3 |
| EC Number | 1.14.14.87 |
| Species | Glycyrrhiza echinata (Licorice) |
| Txid | 46348 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Microsome membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C15H12O4 + C17H21N4O9P-2 + O2 → C15H12O5 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH2 |
| RheaID | 31723 |
+
+
CYP93C2
+
+
+
Substrate Information:
| Substrate Name | Liquiritigenin |
| Substrate Chemical Formula | C15H12O4 |
| Substrate PubChem CID | 114829 |
| Substrate Smiles | C1[C@H](OC2=C(C1=O)C=CC(=C2)O)C3=CC=C(C=C3)O |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | (2R,3S)-2,4′,7-Trihydroxyisoflavanone |
| Product Chemical Formula | C15H12O5 |
| Product PubChem CID | 11459958 |
| Product Smiles | C1=CC(=CC=C1[C@H]2[C@@H](OC3=C(C2=O)C=CC(=C3)O)O)O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice. |
| PMID: | 10557230 |
| Journal: | Plant physiology |
| Year: | 1999/11/1 |
| Title: | Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase. |
| PMID: | 12207646 |
| Journal: | The Plant journal : for cell and molecular biology |
| Year: | 2002/9/1 |
| Title: | Multiple mutagenesis of P450 isoflavonoid synthase reveals a key active-site residue. |
| PMID: | 15809082 |
| Journal: | Biochemical and biophysical research communications |
| Year: | 2005/5/13 |