Detail Information
P450 Protein:
| P450 Symbol | CYP314A1 |
| Protein Name | Ecdysone 20-monooxygenase |
| Uniprot ID | Q9VUF8 |
| EC Number | 1.14.99.22 |
| Gene ID | 39592 |
| Species | Drosophila melanogaster (Fruit fly) |
| Txid | 7227 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Mitochondrion membrane |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C27H44O6 + RH2 + O2 → C27H44O7 + R + H2O |
| Reaction Type | Oxidation |
| Chemical Bond | CH2 |
| RheaID | 14021 |
+
+
CYP314A1
+
+
Substrate Information:
| Substrate Name | Ecdysone |
| Substrate Chemical Formula | C27H44O6 |
| Substrate PubChem CID | 19212 |
| Substrate Smiles | C[C@@H]([C@H]1CC[C@@]2([C@@]1(CC[C@H]3C2=CC(=O)[C@H]4[C@@]3(C[C@@H]([C@@H](C4)O)O)C)C)O)[C@@H](CCC(C)(C)O)O |
| Substrate Structure |
| Substrate Name | AH2 |
| Substrate Chemical Formula | RH2 |
| Substrate Smiles | *([H])[H] |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | 20-Hydroxyecdysone |
| Product Chemical Formula | C27H44O7 |
| Product PubChem CID | 5459840 |
| Product Smiles | C[C@]12CC[C@H]3C(=CC(=O)[C@H]4[C@@]3(C[C@@H]([C@@H](C4)O)O)C)[C@@]1(CC[C@@H]2[C@](C)([C@@H](CCC(C)(C)O)O)O)O |
| Product Structure |
| Product Name | A |
| Product Chemical Formula | R |
| Product Smiles | * |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
References:
| Title: | Shade is the Drosophila P450 enzyme that mediates the hydroxylation of ecdysone to the steroid insect molting hormone 20-hydroxyecdysone. |
| PMID: | 14610274 |
| Journal: | Proceedings of the National Academy of Sciences of the United States of America |
| Year: | 2003/11/25 |