Detail Information
P450 Protein:
| P450 Symbol | tylH1 |
| Protein Name | 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase |
| Uniprot ID | Q9ZHQ1 |
| EC Number | 1.14.15.34 |
| Species | Streptomyces fradiae (Streptomyces roseoflavus) |
| Txid | 1906 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | / |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C31H51NO9 + Fe2S2 + O2 + H+ → C31H51NO10 + Fe2S2 + H2O |
| Reaction Type | Oxidation |
| Chemical Bond | CH3 |
| RheaID | 24524 |
+
+
+
tylH1
+
+
Substrate Information:
| Substrate Name | 20-Oxo-5-O-Beta-D-Mycaminosyltylonolide |
| Substrate Chemical Formula | C31H51NO9 |
| Substrate PubChem CID | 13042283 |
| Substrate Smiles | CC[C@@H]1[C@H](/C=C(/C=C/C(=O)[C@@H](C[C@@H]([C@@H]([C@H]([C@@H](CC(=O)O1)O)C)O[C@H]2[C@@H]([C@H]([C@@H]([C@H](O2)C)O)N(C)C)O)CC=O)C)C)C |
| Substrate Structure |
| Substrate Name | [2Fe-2S]1+ |
| Substrate Chemical Formula | Fe2S2 |
| Substrate Smiles | S1[Fe]S[Fe+]1 |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
| Substrate Name | H+ |
| Substrate Chemical Formula | H+ |
| Substrate PubChem CID | 1038 |
| Substrate CAS Number | 12408-02-5 |
| Substrate Smiles | [H+] |
| Substrate Structure |
Product Information:
| Product Name | 5-O-Beta-D-Mycaminosyltylonolide |
| Product Chemical Formula | C31H51NO10 |
| Product PubChem CID | 6437420 |
| Product Smiles | CC[C@@H]1[C@H](/C=C(/C=C/C(=O)[C@@H](C[C@@H]([C@@H]([C@H]([C@@H](CC(=O)O1)O)C)O[C@H]2[C@@H]([C@H]([C@@H]([C@H](O2)C)O)N(C)C)O)CC=O)C)C)CO |
| Product Structure |
| Product Name | [2Fe-2S]2+ |
| Product Chemical Formula | Fe2S2 |
| Product Smiles | S1[Fe+]S[Fe+]1 |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
References:
| Title: | Properties of Streptomyces fradiae mutants blocked in biosynthesis of the macrolide antibiotic tylosin. |
| PMID: | 7283418 |
| Journal: | Antimicrobial agents and chemotherapy |
| Year: | 1981/8/1 |