Detail Information
P450 Protein:
| P450 Symbol | CYP94C1 |
| Protein Name | Cytochrome P450 94C1 |
| Uniprot ID | Q9ZUX1 |
| EC Number | 1.14.14.49 |
| Gene ID | 817315 |
| Species | Arabidopsis thaliana (Mouse-ear cress) |
| Txid | 3702 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | Membrane; Single-pass membrane protein Endoplasmic reticulum membrane; Single-pass membrane protein |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C14H19NO5R- + C17H21N4O9P-2 + O2 → C14H16NO6R-2 + C17H18N4O9P-3 + H2O + H+ |
| Reaction Type | Oxidation |
| Chemical Bond | CH2-OH |
| RheaID | 55056 |
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+
CYP94C1
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+
Substrate Information:
| Substrate Name | A 12-Hydroxyjasmonyl-L-Alpha-Amino Acid |
| Substrate Chemical Formula | C14H19NO5R- |
| Substrate Smiles | C1(C(CCC1=O)CC(N[C@H](C([O-])=O)*)=O)C/C=CCCO |
| Substrate Structure |
| Substrate Name | FMNH2 |
| Substrate Chemical Formula | C17H21N4O9P-2 |
| Substrate PubChem CID | 44229161 |
| Substrate Smiles | CC1=CC2=C(C=C1C)N(C3=C(N2)C(=O)NC(=O)N3)C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
Product Information:
| Product Name | A 12-Hydroxy-12-Oxojasmonyl-L-Alpha-Amino Acid |
| Product Chemical Formula | C14H16NO6R-2 |
| Product PubChem SID | 85338168 |
| Product Smiles | C1(C(CCC1=O)CC(N[C@H](C([O-])=O)*)=O)C/C=CCC([O-])=O |
| Product Structure |
| Product Name | FMN |
| Product Chemical Formula | C17H18N4O9P-3 |
| Product PubChem CID | 44229199 |
| Product Smiles | CC1=CC2=C(C=C1C)N(C3=NC(=NC(=O)C3=N2)[O-])C[C@@H]([C@@H]([C@@H](COP(=O)([O-])[O-])O)O)O |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
| Product Name | H+ |
| Product Chemical Formula | H+ |
| Product PubChem CID | 1038 |
| Product CAS Number | 12408-02-5 |
| Product Smiles | [H+] |
| Product Structure |
References:
| Title: | Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone Jasmonoyl-isoleucine for catabolic turnover. |
| PMID: | 22215670 |
| Journal: | The Journal of biological chemistry |
| Year: | 2012/2/24 |
| Title: | Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates. |
| PMID: | 26164240 |
| Journal: | Phytochemistry |
| Year: | 2015/9/1 |