Detail Information
P450 Protein:
| P450 Symbol | CYP199A4 |
| Protein Name | CYP199A4 variant F182G |
| Uniprot ID | |
| Species | Rhodopseudomonas palustris (strain HaA2) |
| Txid | 316058 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
| Remarks | Mutations versus WT:F182G |
Reaction Scheme:
| Chemical Conversion | C8H8O3 → C7H6O3 |
| Reaction Type | Oxidation |
| Chemical Bond | O-CH3 |
| Solvents | Tris-HCl buffer, H2O2 |
| Conditions | PH 7.4,30°C |
CYP199A4
Substrate Information:
| Substrate Name | 4-Methoxybenzoic Acid |
| Substrate Chemical Formula | C8H8O3 |
| Substrate PubChem CID | 7478 |
| Substrate Smiles | COC1=CC=C(C=C1)C(=O)O |
| Substrate Structure |
Product Information:
| Product Name | 4-Hydroxybenzoic Acid |
| Product Chemical Formula | C7H6O3 |
| Product PubChem CID | 135 |
| Product CAS Number | 99-96-7 |
| Product Smiles | C1=CC(=CC=C1C(=O)O)O |
| Product Structure |
References:
| Title: | Enabling Peroxygenase Activity in Cytochrome P450 Monooxygenases by Engineering Hydrogen Peroxide Tunnels. |
| PMID: | 36790023 |
| Journal: | Journal of the American Chemical Society |
| Year: | 2023/2/15 |