Detail Information
P450 Protein:
| P450 Symbol | CYP153A |
| Protein Name | Variant V456A |
| Uniprot ID | |
| Species | Marinobacter aquaeolei VT8 |
| Txid | 351348 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
| Remarks | Mutations versus WT:V456A |
Reaction Scheme:
| Chemical Conversion | C12H24O2 → C12H24O3 |
| Reaction Type | Oxidation |
| Chemical Bond | CH3 |
| Solvents | Phosphate buffer,H2O2 |
| Conditions | PH 7.4,30°C |
CYP153A
Substrate Information:
| Substrate Name | Lauric Acid |
| Substrate Chemical Formula | C12H24O2 |
| Substrate PubChem CID | 3893 |
| Substrate Smiles | CCCCCCCCCCCC(=O)O |
| Substrate Structure |
Product Information:
| Product Name | 12-Hydroxydodecanoic Acid |
| Product Chemical Formula | C12H24O3 |
| Product PubChem CID | 79034 |
| Product Smiles | C(O)CCCCCCCCCCC(=O)O |
| Product Structure |
References:
| Title: | Enabling Peroxygenase Activity in Cytochrome P450 Monooxygenases by Engineering Hydrogen Peroxide Tunnels. |
| PMID: | 36790023 |
| Journal: | Journal of the American Chemical Society |
| Year: | 2023/2/15 |