Detail Information
P450 Protein:
| P450 Symbol | CYP105D18 |
| Protein Name | Cytochrome P450 hydroxylase |
| Uniprot ID | |
| Species | Escherichia coli |
| Txid | 595495 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C8H7N → C8H7NO + C16H10N2O2 |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Solvents | Water |
| Conditions | 20 min, 30°C |
CYP105D18
+
Substrate Information:
| Substrate Name | Indole |
| Substrate Chemical Formula | C8H7N |
| Substrate PubChem CID | 789 |
| Substrate Smiles | C=1C=CC=2NC=CC2C1 |
| Substrate Structure |
Product Information:
| Product Name | Indoxyl |
| Product Chemical Formula | C8H7NO |
| Product PubChem CID | 50591 |
| Product CAS Number | 480-93-3 |
| Product Smiles | OC1=CNC=2C=CC=CC12 |
| Product Structure |
| Product Name | Indigo (natural dye) |
| Product Chemical Formula | C16H10N2O2 |
| Product PubChem CID | 10215 |
| Product CAS Number | 68651-46-7 |
| Product Smiles | C1=CC=C2C(=C1)C(=C(N2)C3=NC4=CC=CC=C4C3=O)O |
| Product Structure |
References:
| Title: | In-vitro optimization and active-site mutagenesis of CYP105D18 peroxygenase enhance the production of indigo. |
| PMID: | 40121950 |
| Journal: | Enzyme and Microbial Technology |
| Year: | 2025/4/15 |