Detail Information
P450 Protein:
| P450 Symbol | |
| Protein Name | PikC D50N–RhFRED |
| Uniprot ID | |
| Species | Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD 04745) |
| Txid | 54571 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
| Remarks | Self-sufficient PikC D50N variant C-terminally fused to the RhFRED reductase domain. |
Reaction Scheme:
| Chemical Conversion | C20H39NO3 → C20H38NO4 |
| Reaction Type | Oxidation |
| Chemical Bond | CH2 |
| Conditions | 3 h, pH 7.3, 30°C |
Substrate Information:
| Substrate Name | (2S,3R,4S)-2-(cyclododecyloxy)-4-(dimethylamino)-6-methyltetrahydropyran-3-ol |
| Substrate Chemical Formula | C20H39NO3 |
| Substrate Smiles | C1CCCCC(O[C@@H]2OC(C)C[C@H](N(C)C)[C@H]2O)CCCCCC1 |
| Substrate Structure |
Product Information:
| Product Name | (2S,3R,4S)-4-(dimethylamino)-2-[(7-hydroxycyclododecyl)oxy]-6-methyltetrahydropyran-3-ol |
| Product Chemical Formula | C20H38NO4 |
| Product Smiles | C1CCCCC(O[C@@H]2OC(C)C[C@H](N(C)C)[C@H]2O)CCCCCC1O |
| Product Structure |
References:
| Title: | Selective oxidation of carbolide C-H bonds by an engineered macrolide P450 mono-oxygenase. |
| PMID: | 19833867 |
| Journal: | Proceedings of the National Academy of Sciences of the United States of America (2009) |
| Year: | 2009/10/15 |