Detail Information
P450 Protein:
| P450 Symbol | CYP28 |
| Protein Name | Pentalenic acid synthase |
| Uniprot ID | Q825I8 |
| EC Number | 1.14.15.11 |
| Species | Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) |
| Txid | 227882 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C15H10O5 → C15H10O6 |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Solvents | Methanol, Dimethyl sulfoxide, Water |
| Conditions | 24 h, pH 7.2, 28°C |
| Transformations | 1. Hydroxylation of Aromatic Compounds2. Hydroxylation at an Aromatic Carbon |
CYP28
Substrate Information:
| Substrate Name | genistein |
| Substrate Chemical Formula | C15H10O5 |
| Substrate PubChem CID | 5280961 |
| Substrate Smiles | C1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O |
| Substrate Structure |
Product Information:
| Product Name | Orobol |
| Product Chemical Formula | C15H10O6 |
| Product PubChem CID | 5281801 |
| Product CAS Number | 480-23-9 |
| Product Smiles | C1=CC(=C(C=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O)O |
| Product Structure |
References:
| Title: | Regioselective hydroxylation of isoflavones by Streptomyces avermitilis MA-4680. |
| PMID: | 19577190 |
| Journal: | Journal of Bioscience and Bioengineering (2009) |
| Year: | 2009/2/21 |