Detail Information
P450 Protein:
| P450 Symbol | CYP102A1 |
| Protein Name | P450BM-3 QM |
| Uniprot ID | |
| Species | Priestia megaterium (strain ATCC 14581 / DSM 32 / CCUG 1817 / JCM 2506 / NBRC 15308 / NCIMB 9376 / NCTC 10342 / NRRL B-14308 / VKM B-512 / Ford 19) |
| Txid | 1348623 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
| Remarks | Mutations versus WT:V26T/R47F/A74G/F87V/L188K |
Reaction Scheme:
| Chemical Conversion | C10H16 → C10H16O + C10H16O + C10H16O |
| Reaction Type | Oxidation |
| Chemical Bond | C=C |
| Solvents | Water, Diisononyl phthalate; |
| Conditions | 4 h, pH 7.2, 37°C |
| Transformations | 1. Epoxidation of Alkenes2. Hydroxylation at an Aliphatic Carbon |
CYP102A1
+
+
Substrate Information:
| Substrate Name | ALPHA-PINENE |
| Substrate Chemical Formula | C10H16 |
| Substrate PubChem CID | 6654 |
| Substrate Smiles | CC1=CCC2CC1C2(C)C |
| Substrate Structure |
Product Information:
| Product Name | alpha-pinene oxide |
| Product Chemical Formula | C10H16O |
| Product PubChem CID | 91508 |
| Product CAS Number | 1686-14-2 |
| Product Smiles | CC1(C2CC1C3(C(C2)O3)C)C |
| Product Structure |
| Product Name | verbenol |
| Product Chemical Formula | C10H16O |
| Product PubChem CID | 61126 |
| Product CAS Number | 473-67-6 |
| Product Smiles | CC1=CC(C2CC1C2(C)C)O |
| Product Structure |
| Product Name | Myrtenol |
| Product Chemical Formula | C10H16O |
| Product PubChem CID | 10582 |
| Product CAS Number | 515-00-4 |
| Product Smiles | CC1(C2CC=C(C1C2)CO)C |
| Product Structure |
References:
| Title: | P450(BM-3)-catalyzed whole-cell biotransformation of alpha-pinene with recombinant Escherichia coli in an aqueous-organic two-phase system. |
| PMID: | 19266193 |
| Journal: | Applied Microbiology and Biotechnology (2009) |
| Year: | 2009/3/6 |