Detail Information
P450 Protein:
| P450 Symbol | psmA |
| Protein Name | Cytochrome P450 monooxygenase |
| Uniprot ID | B3XWK3 |
| Species | Streptomyces sp. A-1544 |
| Txid | 347961 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C30H48O8 → C30H48O9 |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Solvents | Dimethyl sulfoxide, Water |
| Conditions | 24 h, pH 6.5, 28°C |
| Transformations | 1. Hydroxylation at an Aliphatic Carbon |
psmA
Substrate Information:
| Substrate Name | pladienolide B |
| Substrate Chemical Formula | C30H48O8 |
| Substrate PubChem CID | 16202130 |
| Substrate Smiles | CCC(C(C)C1C(O1)CC(C)C=CC=C(C)C2C(C=CC(C(CCC(CC(=O)O2)O)(C)O)OC(=O)C)C)O |
| Substrate Structure |
Product Information:
| Product Name | Pladienolide D |
| Product Chemical Formula | C30H48O9 |
| Product PubChem CID | 10007797 |
| Product CAS Number | 445487-91-2 |
| Product Smiles | CCC(C(C)C1C(O1)CC(C)(C=CC=C(C)C2C(C=CC(C(CCC(CC(=O)O2)O)(C)O)OC(=O)C)C)O)O |
| Product Structure |
References:
| Title: | One-pot fermentation of pladienolide D by Streptomyces platensis expressing a heterologous cytochrome P450 gene. |
| PMID: | 19447333 |
| Journal: | Journal of Bioscience and Bioengineering (2009) |
| Year: | 2009/1/23 |