Detail Information
P450 Protein:
| P450 Symbol | vdh |
| Protein Name | Valine dehydrogenase |
| Uniprot ID | Q06539 |
| EC Number | 1.4.1.23 |
| Gene ID | 41989446 |
| Species | Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) |
| Txid | 100226 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C27H44O → C27H44O3 |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Conditions | 3 h, rt |
| Transformations | 1. Hydroxylation at an Aliphatic Carbon |
vdh
Substrate Information:
| Substrate Name | Vitamin D3 |
| Substrate Chemical Formula | C27H44O |
| Substrate PubChem CID | 5280795 |
| Substrate Smiles | CC(C)CCCC(C)C1CCC2C1(CCCC2=CC=C3CC(CCC3=C)O)C |
| Substrate Structure |
Product Information:
| Product Name | calcitriol |
| Product Chemical Formula | C27H44O3 |
| Product PubChem CID | 5280453 |
| Product CAS Number | 32222-06-3 |
| Product Smiles | CC(CCCC(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CC(C3=C)O)O)C |
| Product Structure |
References:
| Title: | Structural evidence for enhancement of sequential vitamin D3 hydroxylation activities by directed evolution of cytochrome P450 vitamin D3 hydroxylase. |
| PMID: | 20667833 |
| Journal: | International Conference on Cytochrome P450 |
| Year: | 2010/7/27 |