Detail Information
P450 Protein:
| P450 Symbol | CYP101A1 |
| Protein Name | Camphor 5-monooxygenase |
| Uniprot ID | P00183 |
| EC Number | 1.14.15.1 |
| Gene ID | 9617760 |
| Species | Pseudomonas putida |
| Txid | 303 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C10H16O + Fe2S2 + O2 + H+ → C10H16O2 + Fe2S2 + H2O |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Transformations | 1. Hydroxylation at an Aliphatic Carbon |
+
+
+
CYP101A1
+
+
Substrate Information:
| Substrate Name | (+)-Camphor |
| Substrate Chemical Formula | C10H16O |
| Substrate PubChem CID | 159055 |
| Substrate Smiles | CC1(C2CCC1(C(=O)C2)C)C |
| Substrate Structure |
| Substrate Name | [2Fe-2S]1+ |
| Substrate Chemical Formula | Fe2S2 |
| Substrate Smiles | S1[Fe]S[Fe+]1 |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
| Substrate Name | H+ |
| Substrate Chemical Formula | H+ |
| Substrate PubChem CID | 1038 |
| Substrate CAS Number | 12408-02-5 |
| Substrate Smiles | [H+] |
| Substrate Structure |
Product Information:
| Product Name | (1R,4R,5R)-5-Hydroxy-1,7,7-trimethylbicyclo[2.2.1]heptan-2-one |
| Product Chemical Formula | C10H16O2 |
| Product PubChem CID | 447414 |
| Product CAS Number | 1607-84-7 |
| Product Smiles | CC1(C2CC(=O)C1(CC2O)C)C |
| Product Structure |
| Product Name | [2Fe-2S]2+ |
| Product Chemical Formula | Fe2S2 |
| Product Smiles | S1[Fe+]S[Fe+]1 |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
References:
| Title: | Molecular recognition in cytochrome P-450: alteration of regioselective alkane hydroxylation via protein engineering |
| DOI: | https://doi.org/10.1021/ja00189a057 |
| Journal: | Journal of the American Chemical Society |
| Year: | 1989/3/1 |