Detail Information

P450 Protein:

P450 SymbolCYP101A1
Protein NameCamphor 5-monooxygenase
Uniprot IDP00183
EC Number1.14.15.1
Gene ID 9617760
SpeciesPseudomonas putida
Txid 303
P450 Protein Structure
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
Powered by PDBe Molstar & AlphaFold
Subcellular Location
Protein Sequence GO

Reaction Scheme:

Chemical Conversion C7H10O → C7H10O2 + C7H10O2 + C7H10O2
Reaction TypeOxidation
Chemical BondCH2

(-)-Norcamphor

CYP101A1

Bicyclo[2.2.1]heptan-2-one, 3-hydroxy-, (1R-exo)-

+

Bicyclo[2.2.1]heptan-2-one, 6-hydroxy-, (1S-exo)-

+

(1S,4S,5R)-5-Hydroxybicyclo[2.2.1]heptan-2-one

Substrate Information:

Substrate Name (-)-Norcamphor
Substrate Chemical FormulaC7H10O
Substrate PubChem CID449588
Substrate SmilesC1CC2CC1CC2=O
Substrate Structure

Product Information:

Product Name Bicyclo[2.2.1]heptan-2-one, 3-hydroxy-, (1R-exo)-
Product Chemical FormulaC7H10O2
Product PubChem CID10887906
Product CAS Number119565-95-6
Product SmilesO=C1C(O)C2CCC1C2
Product Structure
Product Name Bicyclo[2.2.1]heptan-2-one, 6-hydroxy-, (1S-exo)-
Product Chemical FormulaC7H10O2
Product PubChem CID101920744
Product CAS Number114529-16-7
Product SmilesO=C1CC2CC(O)C1C2
Product Structure
Product Name (1S,4S,5R)-5-Hydroxybicyclo[2.2.1]heptan-2-one
Product Chemical FormulaC7H10O2
Product PubChem CID13974584
Product CAS Number114529-14-5
Product SmilesC1C2CC(C1CC2=O)O
Product Structure

References:

Title: Molecular recognition in cytochrome P-450: alteration of regioselective alkane hydroxylation via protein engineering
DOI: https://doi.org/10.1021/ja00189a057
Journal: Journal of the American Chemical Society
Year: 1989/3/1

Contact zhy1001@alu.uestc.edu.cn or yangzhang@cdutcm.edu.cn
© Department of Bioinformatics