Detail Information
P450 Protein:
| P450 Symbol | CYP107A1 |
| Protein Name | 6-deoxyerythronolide B hydroxylase |
| Uniprot ID | Q00441 |
| EC Number | 1.14.15.35 |
| Species | Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338) |
| Txid | 405948 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C9H10 + C4H6N2O2 → C13H16O2 + N2 |
| Reaction Type | Oxidation |
| Chemical Bond | |
| Conditions | 20 μM P450, 5% MeOH,10 mM Na2S2O4,0.1 M KPi, pH 8.0,argon, rt, 16h |
+
CYP107A1
+
Substrate Information:
| Substrate Name | Alpha-Methylstyrene |
| Substrate Chemical Formula | C9H10 |
| Substrate PubChem CID | 7407 |
| Substrate Smiles | C1=CC=CC=C1C(C)=C |
| Substrate Structure |
| Substrate Name | Ethyl diazoacetate |
| Substrate Chemical Formula | C4H6N2O2 |
| Substrate PubChem CID | 12192 |
| Substrate CAS Number | 623-73-4 |
| Substrate Smiles | [N-]=[N+]=CC(=O)OCC |
| Substrate Structure |
Product Information:
| Product Name | ethyl (1S,2S)-2-methyl-2-phenylcyclopropane-1-carboxylate |
| Product Chemical Formula | C13H16O2 |
| Product Smiles | [C@@](C)(C1=CC=CC=C1)1C[C@@H]1C(OCC)=O |
| Product Structure |
| Product Name | Nitrogen |
| Product Chemical Formula | N2 |
| Product PubChem CID | 947 |
| Product CAS Number | 7727-37-9 |
| Product Smiles | N#N |
| Product Structure |
References:
| Title: | Mutating a Highly Conserved Residue in Diverse Cytochrome P450s Facilitates Diastereoselective Olefin Cyclopropanation |
| PMID: | 26690878 |
| Journal: | Chembiochem : a European journal of chemical biology |
| Year: | 2016/3/2 |