Detail Information
P450 Protein:
| P450 Symbol | Cgl0553 |
| Protein Name | Cytochrome P450 |
| Uniprot ID | Q8NSW2 |
| Species | Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) |
| Txid | 196627 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
| Remarks | P450 monooxygenase CreJ, when partnered with the native redox partner proteins including ferredoxin CreF and ferredoxin reductase CreE |
Reaction Scheme:
| Chemical Conversion | C7H8O + C21H26N7O17P3-4 + O2 + H+ → C7H8O2 + C21H25N7O17P3-3 + H2O |
| Reaction Type | Oxidation |
| Chemical Bond | CH3 |
| Conditions | 2 h, 30°C |
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Cgl0553
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Substrate Information:
| Substrate Name | P-Cresol |
| Substrate Chemical Formula | C7H8O |
| Substrate PubChem CID | 2879 |
| Substrate CAS Number | 106-44-5 |
| Substrate Smiles | CC1=CC=C(C=C1)O |
| Substrate Structure |
| Substrate Name | NADPH(4-) |
| Substrate Chemical Formula | C21H26N7O17P3-4 |
| Substrate PubChem CID | 15983949 |
| Substrate Smiles | C1C=CN(C=C1C(=O)N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)([O-])OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)OP(=O)([O-])[O-])O)O)O |
| Substrate Structure |
| Substrate Name | O2 |
| Substrate Chemical Formula | O2 |
| Substrate PubChem CID | 977 |
| Substrate CAS Number | 7782-44-7 |
| Substrate Smiles | O=O |
| Substrate Structure |
| Substrate Name | H+ |
| Substrate Chemical Formula | H+ |
| Substrate PubChem CID | 1038 |
| Substrate CAS Number | 12408-02-5 |
| Substrate Smiles | [H+] |
| Substrate Structure |
Product Information:
| Product Name | 4-Hydroxybenzyl Alcohol |
| Product Chemical Formula | C7H8O2 |
| Product PubChem CID | 125 |
| Product CAS Number | 623-05-2 |
| Product Smiles | C1=CC(=CC=C1CO)O |
| Product Structure |
| Product Name | NADP+ |
| Product Chemical Formula | C21H25N7O17P3-3 |
| Product PubChem CID | 162422398 |
| Product Smiles | C1=CC(=C[N+](=C1)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)([O-])OP(=O)([O-])OC[C@@H]3[C@H]([C@H]([C@@H](O3)N4C=NC5=C(N=CN=C54)N)O)OP(=O)([O-])[O-])O)O)C(=O)N |
| Product Structure |
| Product Name | H2O |
| Product Chemical Formula | H2O |
| Product PubChem CID | 962 |
| Product CAS Number | 7732-18-5 |
| Product Smiles | O |
| Product Structure |
References:
| Title: | Selective oxidation of aliphatic C-H bonds in alkylphenols by a chemomimetic biocatalytic system. |
| PMID: | 28607077 |
| Journal: | Proceedings of the National Academy of Sciences of the United States of America |
| Year: | 2017/6/27 |