Detail Information
P450 Protein:
| P450 Symbol | juvC |
| Protein Name | Cytochrome P450 hydroxylase |
| Uniprot ID | A0A1Z1MZ61 |
| Species | Micromonospora chalcea subsp. izumensis |
| Txid | 2008351 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C31H51NO8 → C31H51NO9 |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Solvents | Dimethyl sulfoxide;Water |
| Conditions | overnight, pH 7.3, 30°C |
| Transformations | Hydroxylation at an Aliphatic Carbon |
juvC
Substrate Information:
| Substrate Name | Cirramycin A1, 12,13-deepoxy-12,13-didehydro-4-deoxy-, (12E)- (9CI, ACI) |
| Substrate Chemical Formula | C31H51NO8 |
| Substrate PubChem CID | 41968 |
| Substrate Smiles | O=CCC1CC(C(=O)C=CC(=CC(C)C(OC(=O)CC(O)C(C)C1OC2OC(C)CC(N(C)C)C2O)CC)C)C |
| Substrate Structure |
Product Information:
| Product Name | Tylosin, 23-O-de(6-deoxy-2,3-di-O-methyl-.beta.-D-allopyranosyl)-4A-O-de(2,6-dideoxy-3-C-methyl-alpha-L-ribo-hexopyranosyl)-20-deoxo- (ACI) |
| Product Chemical Formula | C31H51NO9 |
| Product PubChem CID | 78410181 |
| Product CAS Number | 80240-61-5 |
| Product Smiles | O=CCC1CC(C(=O)C=CC(=CC(CO)C(OC(=O)CC(O)C(C)C1OC2OC(C)CC(N(C)C)C2O)CC)C)C |
| Product Structure |
References:
| Title: | Chemoenzymatic Total Synthesis and Structural Diversification of Tylactone-Based Macrolide Antibiotics through Late-Stage Polyketide Assembly, Tailoring, and C-H Functionalization. |
| PMID: | 28525276 |
| Journal: | Journal of the American Chemical Society |
| Year: | 2017/6/5 |