Detail Information

P450 Protein:

P450 Symbol	juvD
Protein Name	Cytochrome P450 epoxidase
Uniprot ID	A0A1Z1MZ59
Species	Micromonospora chalcea subsp. izumensis
Txid	2008351
P450 Protein Structure	Go to AlphaFold Website Model Confidence (pLDDT) Very high (>90) High (90 > 70) Low (70 > 50) Very low (<50) Powered by PDBe Molstar & AlphaFold
Subcellular Location
Protein Sequence	GO

Reaction Scheme:

Chemical Conversion	C31H51NO8 → C31H51NO9
Reaction Type	Oxidation
Chemical Bond	CH
Solvents	Dimethyl sulfoxide;Water
Conditions	overnight, pH 7.3, 30°C
Transformations	Hydroxylation at an Aliphatic Carbon

Cirramycin A1, 12,13-deepoxy-12,13-didehydro-4-deoxy-, (12E)- (9CI, ACI)

juvD

Tylosin, 23-O-de(6-deoxy-2,3-di-O-methyl-.beta.-D-allopyranosyl)-4A-O-de(2,6-dideoxy-3-C-methyl-alpha-L-ribo-hexopyranosyl)-20-deoxo- (ACI)

Substrate Information:

Substrate Name	Cirramycin A1, 12,13-deepoxy-12,13-didehydro-4-deoxy-, (12E)- (9CI, ACI)
Substrate Chemical Formula	C31H51NO8
Substrate PubChem CID	41968
Substrate Smiles	O=CCC1CC(C(=O)C=CC(=CC(C)C(OC(=O)CC(O)C(C)C1OC2OC(C)CC(N(C)C)C2O)CC)C)C
Substrate Structure	Download SDF

Product Information:

Product Name	Tylosin, 23-O-de(6-deoxy-2,3-di-O-methyl-.beta.-D-allopyranosyl)-4A-O-de(2,6-dideoxy-3-C-methyl-alpha-L-ribo-hexopyranosyl)-20-deoxo- (ACI)
Product Chemical Formula	C31H51NO9
Product PubChem CID	78410181
Product CAS Number	80240-61-5
Product Smiles	O=CCC1CC(C(=O)C=CC(=CC(CO)C(OC(=O)CC(O)C(C)C1OC2OC(C)CC(N(C)C)C2O)CC)C)C
Product Structure	Download SDF

References:

Title:	Chemoenzymatic Total Synthesis and Structural Diversification of Tylactone-Based Macrolide Antibiotics through Late-Stage Polyketide Assembly, Tailoring, and C-H Functionalization.
PMID:	28525276
Journal:	Journal of the American Chemical Society
Year:	2017/6/5