Detail Information
P450 Protein:
| P450 Symbol | CYP105A1 |
| Protein Name | Vitamin D3 dihydroxylase |
| Uniprot ID | |
| Species | Streptomyces griseolus |
| Txid | 1909 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
| Remarks | The R73V/R84A variant of CYP105A1 |
Reaction Scheme:
| Chemical Conversion | C27H44O3 → C27H44O4 |
| Reaction Type | Oxidation |
| Chemical Bond | CH3 |
| Solvents | Ethanol;Water |
| Conditions | 30°C |
| Transformations | Hydroxylation at an Aliphatic Carbon |
CYP105A1
Substrate Information:
| Substrate Name | Calcitriol |
| Substrate Chemical Formula | C27H44O3 |
| Substrate PubChem CID | 5280453 |
| Substrate CAS Number | 32222-06-3 |
| Substrate Smiles | C[C@@]12[C@](C(=CC=C3/C(=C)[C@@H](O)C[C@H](O)C3)CCC1)(CC[C@@]2([C@@H](CCCC(C)(C)O)C)[H])[H] |
| Substrate Structure |
Product Information:
| Product Name | 1,25,26-Trihydroxyvitamin D3 |
| Product Chemical Formula | C27H44O4 |
| Product PubChem CID | 5283758 |
| Product CAS Number | 77372-59-9 |
| Product Smiles | C[C@@]12[C@](C(=CC=C3/C(=C)[C@@H](O)C[C@H](O)C3)CCC1)(CC[C@@]2([C@@H](CCC[C@](CO)(C)O)C)[H])[H] |
| Product Structure |
References:
| Title: | Three-step hydroxylation of vitamin D3 by a genetically engineered CYP105A1: enzymes and catalysis. |
| PMID: | 20731719 |
| Journal: | FEBS Journal |
| Year: | 2010/8/20 |