Detail Information
P450 Protein:
| P450 Symbol | CYP153A7 |
| Protein Name | P450pyrSM2 |
| Uniprot ID | |
| Species | Sphingopyxis macrogoltabida |
| Txid | 33050 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
| Remarks | This reaction was catalyzed by P450pyrSM2, an engineered sextuple mutant of P450pyr hydroxylase generated by directed evolution. The mutant contains the substitutions I83F, N100S, T186I, L251V, L302V, and F403I. |
Reaction Scheme:
| Chemical Conversion | C9H12 → C9H12O |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Solvents | Water |
| Conditions | 4 h, 30°C |
| Transformations | Hydroxylation at an Aliphatic Carbon |
CYP153A7
Substrate Information:
| Substrate Name | Propylbenzene |
| Substrate Chemical Formula | C9H12 |
| Substrate PubChem CID | 7668 |
| Substrate Smiles | C=1C=CC(=CC1)CCC |
| Substrate Structure |
Product Information:
| Product Name | (S)-1-Phenyl-2-propanol |
| Product Chemical Formula | C9H12O |
| Product PubChem CID | 6994174 |
| Product CAS Number | 1517-68-6 |
| Product Smiles | C([C@H](C)O)C1=CC=CC=C1 |
| Product Structure |
References:
| Title: | Engineering of p450pyr hydroxylase for the highly regio- and enantioselective subterminal hydroxylation of alkanes. |
| PMID: | 24554642 |
| Journal: | Angewandte Chemie, International Edition |
| Year: | 2014/2/19 |