Detail Information
P450 Protein:
| P450 Symbol | CYP716C49 |
| Protein Name | Triterpene C-2alpha hydroxylase |
| Uniprot ID | |
| Species | Crataegus pinnatifida |
| Txid | 510735 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C30H48O3 → C30H48O4 |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Transformations | Hydroxylation at an Aliphatic Carbon |
CYP716C49
Substrate Information:
| Substrate Name | (1R,3aS,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR)-9-hydroxy-5a,5b,8,8,11a-pentamethyl-1-prop-1-en-2-yl-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysene-3a-carboxylic acid |
| Substrate Chemical Formula | C30H48O3 |
| Substrate PubChem CID | 64971 |
| Substrate Smiles | CC(=C)[C@@H]1CC[C@]2([C@H]1[C@H]3CC[C@@H]4[C@]5(CC[C@@H](C([C@@H]5CC[C@]4([C@@]3(CC2)C)C)(C)C)O)C)C(=O)O |
| Substrate Structure |
Product Information:
| Product Name | (1R,3aS,5aR,5bR,7aR,9R,10R,11aR,11bR,13aR,13bR)-9,10-dihydroxy-5a,5b,8,8,11a-pentamethyl-1-prop-1-en-2-yl-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysene-3a-carboxylic acid |
| Product Chemical Formula | C30H48O4 |
| Product PubChem CID | 12305768 |
| Product CAS Number | 19533-92-7 |
| Product Smiles | CC(=C)[C@@H]1CC[C@]2([C@H]1[C@H]3CC[C@H]4[C@]([C@@]3(CC2)C)(CC[C@@H]5[C@@]4(C[C@H]([C@@H](C5(C)C)O)O)C)C)C(=O)O |
| Product Structure |
References:
| Title: | Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories. |
| PMID: | 30339834 |
| Journal: | Metabolic Engineering |
| Year: | 2019/1/ |