Detail Information
P450 Protein:
| P450 Symbol | CYP716C49 |
| Protein Name | Triterpene C-2alpha hydroxylase |
| Uniprot ID | |
| Species | Crataegus pinnatifida |
| Txid | 510735 |
| P450 Protein Structure |
Model Confidence (pLDDT)
Very high (>90)
High (90 > 70)
Low (70 > 50)
Very low (<50)
|
| Subcellular Location | |
| Protein Sequence | GO |
Reaction Scheme:
| Chemical Conversion | C30H48O3 → C30H48O4 |
| Reaction Type | Oxidation |
| Chemical Bond | CH |
| Transformations | Hydroxylation at an Aliphatic Carbon |
CYP716C49
Substrate Information:
| Substrate Name | (4aS,6aR,6aS,6bR,8aR,10S,12aR,14bS)-10-hydroxy-2,2,6a,6b,9,9,12a-heptamethyl-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-4a-carboxylic acid |
| Substrate Chemical Formula | C30H48O3 |
| Substrate PubChem CID | 10494 |
| Substrate Smiles | C[C@]12CC[C@@H](C([C@@H]1CC[C@@]3([C@@H]2CC=C4[C@]3(CC[C@@]5([C@H]4CC(CC5)(C)C)C(=O)O)C)C)(C)C)O |
| Substrate Structure |
Product Information:
| Product Name | (4aS,6aR,6aS,6bR,8aR,10R,11R,12aR,14bS)-10,11-dihydroxy-2,2,6a,6b,9,9,12a-heptamethyl-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-4a-carboxylic acid |
| Product Chemical Formula | C30H48O4 |
| Product PubChem CID | 73659 |
| Product CAS Number | 4373-41-5 |
| Product Smiles | C[C@@]12CC[C@@H]3[C@@]([C@H]1CC=C4[C@]2(CC[C@@]5([C@H]4CC(CC5)(C)C)C(=O)O)C)(C[C@H]([C@@H](C3(C)C)O)O)C |
| Product Structure |
References:
| Title: | Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories. |
| PMID: | 30339834 |
| Journal: | Metabolic Engineering |
| Year: | 2019/1/ |